Engineered endonucleases, such as transcription activator-like effector nucleases (TALENs), facilitate precise genetic modification in animals. At CD BioSciences, our Mammary Gland Bioreactor TALENs Service harnesses the power of TALENs to optimize recombinant protein production in mammary glands, ensuring enhanced efficiency and stability.
Fig.1 Schematic illustration of mode of action of TALENs inducing a double-strand break on the target DNA. (Bhardwaj, A., et al., 2021)
Introduction to TALENs
TALENs are powerful genome-editing tools that have gained popularity due to their ability to induce precise genetic modifications. TALENs consist of customizable DNA-binding domains fused to a DNA cleavage domain, allowing them to target specific genomic sequences with high specificity.
Mechanism of Action
TALENs create double-strand breaks (DSBs) at targeted DNA sites. These DSBs are predominantly repaired by the cell's natural repair mechanism, known as non-homologous end joining (NHEJ). NHEJ is an error-prone process that can introduce small insertions and deletions (indels) at the target site, leading to gene disruptions or alterations.
Precision and Off-Target Effects
Although TALENs exhibit higher specificity and lower off-target effects compared to some other genome-editing tools, off-target events can still occur. To minimize the risk of unwanted gene mutations, certain strategies can be employed. For instance, inducing single-strand breaks (SSBs) or nicks instead of DSBs can significantly reduce off-target effects and improve the accuracy of gene modifications.
TALE Nickases
TALE nickases are modified versions of TALENs designed to introduce SSBs rather than DSBs. This modification enhances the precision of gene editing by promoting homology-directed repair (HDR) over NHEJ. HDR is a more accurate repair mechanism that uses a homologous DNA template to precisely repair the break, leading to more controlled and specific genetic modifications.
Applications in Mammary Gland Bioreactors
TALE nickases hold great potential for producing mammary gland bioreactors, which are used to synthesize and secrete recombinant proteins in the milk of transgenic animals. By targeting the mammary protein locus with high precision, TALE nickases can facilitate the stable and specific integration of foreign genes, ensuring consistent and high-yield production of therapeutic proteins.
Our Services
Our TALENs Service at CD BioSciences is specifically designed to address the challenges of random gene integration and unstable protein expression in mammary gland bioreactor technology. Through precise genome editing, we ensure optimal insertion sites for foreign genes within the genome, thereby enhancing recombinant protein expression stability and consistency.
TALENs Design and Synthesis
Custom design and synthesis of TALENs to target specific genomic sequences for precise gene editing.
Vector Construction
Construction of vectors containing TALENs and foreign genes for efficient gene delivery.
Animal Model Creation
Establishment of animal models with stable gene expression in mammary glands through precise genome editing.
Protein Expression AnalysisProtein Expression Analysis
Evaluation and optimization of recombinant protein expression levels and stability in mammary glands.
Why Choose Us
- High Precision
TALENs provide accurate and specific gene editing capabilities, minimizing off-target effects.
- Versatility
Applicable to a wide range of genetic modifications, including insertions, deletions, and corrections.
- Efficiency
High rates of successful gene editing, leading to reliable production of recombinant proteins.
- Cost-Effective
Reduced production costs compared to traditional cell culture methods, with scalable outputs.
Contact Us
By leveraging our mammary gland bioreactor TALENs service at CD BioSciences, clients can achieve groundbreaking advancements in protein production, ensuring the delivery of high-quality and cost-effective protein products. To obtain further information or discuss your specific project requirements, please do not hesitate to contact us.
Reference
- Bhardwaj, A., & Nain, V. (2021). TALENs-an indispensable tool in the era of CRISPR: a mini review. Journal, genetic engineering & biotechnology, 19(1), 125.
Our services are for research use only and cannot be used for any clinical purposes.
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