The utilization of transgenic animals as mammary gland bioreactors demonstrates significant potential for protein production. However, conventional methods for generating transgenic animals, such as somatic cell nuclear transfer (SCNT), possess limitations in terms of efficiency and labor intensity. The emergence of gene editing technologies, particularly the CRISPR/Cas9 system, provides a more efficient pathway for producing transgenic animals as mammary gland bioreactors.
Introduction to CRISPR/Cas9
The CRISPR/Cas9 system, derived from the immune defense mechanisms of archaea and bacteria, has transformed gene editing.
| Overview |
This technology utilizes engineered single guide RNAs (sgRNAs) to direct the Cas9 protein towards specific genomic sequences, allowing for precise DNA cleavage at desired locations. Compared to previous gene editing tools like transcription activator-like effector nucleases (TALENs), CRISPR/Cas9 offers unparalleled advantages in simplicity, cost-effectiveness, and efficiency. |
| Mechanism |
Following DNA cleavage, two primary repair pathways are activated: non-homologous end joining (NHEJ) and homology-directed repair (HDR). If a foreign DNA donor template is available, HDR permits accurate sequence insertion, while NHEJ may result in unpredictable insertions or deletions. |
| Applications |
This adaptability facilitates precise gene knock-in (KI) or knock-out (KO), making CRISPR/Cas9 indispensable for developing genetically modified animal models and production lines. |
Fig.1 A brief history of CRISPR/Cas9 system development and associated gene editing tools. (Wang, S. W., et al., 2022)
Our Services
At CD BioSciences, we are at the forefront of protein production innovation, employing cutting-edge technologies to push the boundaries of what is achievable. Our CRISPR/Cas9 gene editing service for mammary gland bioreactors harnesses the precision and efficiency of CRISPR/Cas9 technology to enhance recombinant protein production with unparalleled accuracy.
A range of gene editing and modifications can be accomplished using CRISPR/Cas9.
Gene Knockout
Using a guide RNA, CRISPR/Cas9 creates a break at a precise location in the gene. The cell's natural repair processes often result in the addition or deletion of base pairs, effectively disrupting the gene. In mammary gland bioreactors, this method can be utilized to study the effects of gene disruption on milk protein composition and yield.
Gene Knock-in
The guide RNA directs CRISPR/Cas9 to a specific site where a new gene is introduced. This new gene is integrated into the genome during the cell's repair process. In mammary gland bioreactors, gene knock-in can be used to produce high-value recombinant proteins in milk, enhancing the utility of these systems for protein productio.
Workflow of Our Service
Target Gene Construction and Vector Design
Custom design and construction of CRISPR/Cas9 vectors for precise gene editing.
Phenotypic Characterization and Production Optimization
Comprehensive analysis and optimization of protein production in transgenic animals.
Generation of Transgenic Animals
Development of transgenic animals with stable gene integration and expression in mammary glands.
Product Quality Control
Rigorous quality control to ensure the consistency and purity of the produced recombinant proteins.
Contact Us
CD BioSciences offers a comprehensive range of CRISPR/Cas9 services tailored to the specific requirements of mammary gland bioreactor development. Our services prioritize precision and efficiency, ensuring that our knowledge remains up-to-date to advance the frontiers of mammary gland bioreactor research with exceptional expertise. To cater to the diverse needs of this industry, we have established a global network of partnerships, enabling us to provide these capabilities worldwide. If you are interested in availing our services, please feel free to contact us.
Reference
- Wang, S. W., et al.; (2022). Current applications and future perspective of CRISPR/Cas9 gene editing in cancer. Molecular cancer, 21(1), 57.
Our services are for research use only and cannot be used for any clinical purposes.
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