The mammary gland bioreactor is considered a promising approach for producing pharmaceutical proteins. However, one of the challenges in this technique lies in achieving optimal expression levels of the transgene. At CD BioSciences, our mammary gland bioreactor Cre-loxP service utilizes the precision of the Cre-loxP recombination system to enhance recombinant protein production in animal mammary glands.
Introduction to Cre-loxP
The Cre-loxP system is a precise genetic engineering tool derived from bacteriophage P1, used for targeted DNA manipulation. Cre recombinase, an enzyme, recognizes and recombines loxP sites - specific DNA sequences. This enables the insertion, deletion, or rearrangement of DNA segments at specific locations within the genome.
Widely utilized for generating transgenic models and studying gene functions, Cre-loxP allows for controlled gene modifications. In mammary gland bioreactors, it ensures stable and high-level expression of recombinant proteins, making it essential for advancing protein production in biotechnology.
Fig.1 Principles and approaches of inducible Cre-loxP-mediated site-specific recombination. (Wang, T., et al., 2023)
Our Services
At CD BioSciences, we are at the forefront of preclinical contract research organization (CRO) services, specializing in the development and optimization of mammary gland bioreactor technology. Our mammary gland bioreactor Cre-loxP service plays a pivotal role in advancing the bio-pharmaceutical industry by offering high-expression, cost-effective, and structurally authentic proteins.
Design and Integration
- Design the Targeting Construct
We design a loxP-flanked gene construct for targeted expression in mammary glands.
- Introduce the Construct into ES Cells
The construct is introduced into embryonic stem (ES) cells for gene integration.
Validation and Generation
- Identify and Validate ES Cell Clones
We screen for successful integration and select positive ES cell clones.
- Generate Chimeric Animals
Chimeric animals are generated to establish a knock-in animal line.
Recombination and Characterization
- Recombination and Characterization
Cre recombinase induces precise gene recombination in the knock-in line.
- Characterize the Knock-in Model
We verify gene expression and protein production in the mammary glands.
- Recombinant Protein Harvesting
Efficiently harvest high-quality proteins from the milk of transgenic animals.
- Purification and Characterization
Guarantee purity and functionality of synthesized proteins.
Our Advantage
Precision and Control
The Cre-loxP system offers unmatched precision in gene targeting, allowing for controlled and predictable expression of recombinant proteins.
Cost-Effectiveness
Leveraging the natural protein synthesis capabilities of mammary glands reduces production costs compared to traditional cell culture methods.
High Yield and Quality
Our optimized strategies ensure the high-yield production of proteins that closely mimic their natural counterparts in structure and function.
Contact Us
CD BioSciences is committed to advancing mammary gland bioreactor technology through our comprehensive Cre-loxP service. By integrating cutting-edge gene editing techniques, we enhance the stability and expression of recombinant proteins. Contact us to leverage our expertise and elevate your protein production capabilities to new heights.
Reference
- Wang, T., et al.; (2023). Cre-loxP-mediated genetic lineage tracing: Unraveling cell fate and origin in the developing heart. Frontiers in cardiovascular medicine, 10, 1085629.
Our services are for research use only and cannot be used for any clinical purposes.
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