As part of our comprehensive mammary gland bioreactor technology platform, CD BioSciences offers a specialized biological products purification service aimed at ensuring the utmost quality of recombinant proteins produced in mammary glands. The purification process plays a critical role in isolating these proteins from the intricate mixture of components present in milk, guaranteeing that the final product is pure, stable, and biologically active for therapeutic applications.
Our Services
The successful purification of recombinant proteins from mammary gland bioreactors is crucial for their application in research and therapy, which we fully comprehend at CD BioSciences. To ensure the highest standards of purity and functionality, we employ a combination of advanced purification methods tailored to the unique characteristics of each protein.
Purification Methods
To ensure the utmost purity of our recombinant proteins, we employ a diverse array of state-of-the-art purification techniques, carefully selected based on the specific characteristics of the target protein and the impurities present in the milk.
Ion-Exchange Chromatography (IEX)
This technique separates proteins based on their net charge. By carefully adjusting the pH and ionic strength of the buffer, we can selectively bind and elute proteins, achieving high purity and yield.
Affinity Chromatography
Affinity chromatography leverages the specific binding interactions between a protein and a ligand. This method is particularly effective for purifying proteins that have unique binding properties, ensuring that the target protein is isolated with minimal contamination.
Size-Exclusion Chromatography (SEC)
Also known as gel filtration, SEC separates proteins based on their size. This technique is crucial for removing aggregates and ensuring the homogeneity of the final product, which is essential for therapeutic applications.
Hydrophobic Interaction Chromatography (HIC)
HIC exploits the hydrophobic properties of proteins to achieve separation. By altering the salt concentration, we can isolate proteins with different levels of hydrophobicity, helping to refine the purity of the final product.
Purification Workflow
The purification process of recombinant proteins from mammary gland bioreactors involves a multi-step protocol aimed at maximizing yield while preserving protein integrity. Our optimized workflow is specifically designed to handle the complexities associated with milk as the starting material, ensuring the isolation of target proteins in their purest form.
Protein Concentration
Ultrafiltration concentrates proteins, reducing solution volume and removing small impurities.
Final Polishing and Formulation
Hydrophobic interaction chromatography and formulation in a stable buffer achieve the highest purity and stability.
Pre-Purification Clarification
Centrifugation and filtration remove fats, caseins, and other milk components, yielding a clarified supernatant.
Sequential Chromatography
Multiple rounds of chromatography, including ion exchange, affinity, and size-exclusion, purify the target protein.
Quality Assurance
Rigorous testing, including SDS-PAGE and mass spectrometry, ensures the final product meets stringent quality standards
Contact Us
By integrating these sophisticated purification techniques with an optimized workflow, CD BioSciences is capable of delivering recombinant proteins of exceptional quality, thereby facilitating the advancement of biopharmaceutical research and development. Should you require any further assistance, please do not hesitate to
contact us.
Our services are for research use only and cannot be used for any clinical purposes.
Related Solutions
